https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36741 Thu 02 Jul 2020 16:31:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31522 K_d ∼ 0.4 mM). FACE was easily carried out at shorter binding equilibration time (i.e. 30 min) and without the need to separate the microspheres, circumventing laborious and, in the case of the system under study, inefficient sample filtration. It also allowed for competitive binding studies by virtue of its ability to distinctly separate intact microspheres and all tested amines which could not be resolved in HPLC. K_d’s for nonimprinted (control) microspheres (NIM) from FACE and HPLC were also comparable (∼ 0.6 mM) but at higher histamine concentrations, HPLC gave lower histamine binding. This discrepancy was attributed to inefficient filtration of the batch binding samples prior to HPLC analysis resulting in an over-estimation of the concentration of free histamine brought about by the presence of unfiltered histamine-bound microspheres.]]> Sat 24 Mar 2018 08:43:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20719 Sat 24 Mar 2018 08:06:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30246 Sat 24 Mar 2018 07:41:57 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25364 Sat 24 Mar 2018 07:24:40 AEDT ]]>